Article
The EGD1 product, a yeast homolog of human BTF3, may be involved in GAL4 DNA binding.
Molecular and cellular biology - 1 Dec 1992
Parthun M R, Mangus D A, Jaehning J A
Abstract excerpt
A variety of techniques, including filter binding, footprinting, and gel retardation, can be used to assay the transcriptional activator GAL4 (Gal4p) through the initial steps of its purification from yeast cells. Following DNA affinity chromatography, Gal4p still bound DNA selectively when assay...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromatography, Affinity
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- Galactose
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors