Article
Secondary structure and interactions of the packaged dsDNA genome of bacteriophage P22 investigated by Raman difference spectroscopy.
Biochemistry - 1 Dec 1992
Aubrey K L, Casjens S R, Thomas G J
Abstract excerpt
Vibrational spectra of the double-stranded DNA genome of bacteriophage P22 in packaged and unpackaged states are compared by digital difference Raman spectroscopy. The difference Raman spectrum, which is sensitive to structural changes at the level of < 2% of a given nucleotide type, reveals the effects of packaging upon sugar pucker, glycosyl orientation, phosphodiester geometry, base pairing, base stacking, and...
Topics
- Bacteriophages
- Base Composition
- Binding Sites
- DNA, Viral
- Electrochemistry
- Genes, Viral
- Magnesium
- Mutation
- Nucleic Acid Conformation
- Phosphates
- Spectrum Analysis, Raman