Article
Ultrastructural examination of the lipopolysaccharides of Pseudomonas aeruginosa strains and their isogenic rough mutants by freeze-substitution.
Journal of bacteriology - 1 Nov 1992
Lam J S, Graham L L, Lightfoot J, Dasgupta T, Beveridge T J
Abstract excerpt
The majority of Pseudomonas aeruginosa strains synthesize two antigenically distinct types of lipopolysaccharide (LPS), namely, a serotype-specific B-band LPS and a common antigen A-band LPS. A-band LPS consists of uncharged poly-D-rhamnan, which does not bind uranyl ions and is difficult to stain for electron microscopy; the highly charged B-band LPS is more easily visualized. We selected two wild-type strains,...
Topics
- Cell Membrane
- Electrophoresis, Polyacrylamide Gel
- Freezing
- Lipopolysaccharides
- Microscopy, Electron
- Mutation
- Pseudomonas aeruginosa