Article
A single amino-acid change in the iron-sulphur protein subunit of succinate dehydrogenase confers resistance to carboxin in Ustilago maydis.
Current genetics - 1 Aug 1992
Broomfield P L, Hargreaves J A
Abstract excerpt
The sequence of an allele encoding the iron-sulphur protein (Ip) subunit of succinate dehydrogenase (Sdh) was determined following PCR amplification of genomic DNA from a carboxin (Cbx)-sensitive Ustilago maydis strain. Comparison of this sequence with that of the Ip allele from a Cbx-resistant strain (IPr) revealed a two-base difference between the sequences. This mutation led to the substitution of a leucine...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Carboxin
- Drug Resistance, Microbial
- Electron Transport
- Fungal Proteins
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Succinate Dehydrogenase