Article
Development of a LAMP method for detecting the N75S mutant in SDHI-resistant Corynespora cassiicola.
Analytical biochemistry - 15 May 2020
Zhu Jiamei, Zhang Lingyan, Li Hong, Gao Yangyang, Mu Wei, Liu Feng
Abstract excerpt
The replacement of asparagine with serine at codon 75 of the sdhC gene (N75S) confers succinate dehydrogenase inhibitor resistance in Corynespora cassiicola, which caused by consecutive fungicide application. To rapidly detect the mutation of N75S, a method based on loop-mediated isothermal amplification (LAMP) was developed in this study. The optimal primer set among the six primer sets designed could clearly...
Topics
- Ascomycota
- DNA, Fungal
- Drug Resistance, Fungal
- Fungal Proteins
- Molecular Diagnostic Techniques
- Mutation
- Nucleic Acid Amplification Techniques
