Article
A missense mutation of L-gulono-gamma-lactone oxidase causes the inability of scurvy-prone osteogenic disorder rats to synthesize L-ascorbic acid.
The Journal of biological chemistry - 25 Oct 1992
Kawai T, Nishikimi M, Ozawa T, Yagi K
Abstract excerpt
The osteogenic disorder Shionogi (ODS) rat is a mutant Wistar rat that is subject to scurvy, because it lacks L-gulono-gamma-lactone oxidase, a key enzyme in L-ascorbic acid biosynthesis. Sequencing of polymerase chain reaction-amplified cDNAs for mutant and normal rat L-gulono-gamma-lactone oxid...
Topics
- Animals
- Ascorbic Acid
- Base Sequence
- Blotting, Northern
- Cell Line, Transformed
- Cells, Cultured
- DNA
- Genetic Predisposition to Disease
- L-Gulonolactone Oxidase
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- RNA, Messenger
- Rats
- Rats, Mutant Strains
- Scurvy
- Sugar Alcohol Dehydrogenases