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Article

The use of antisense mRNA to inhibit the tonoplast H+ ATPase in carrot.

The Plant cell - 1 Jul 1992

Gogarten J P, Fichmann J, Braun Y, Morgan L, Styles P, Taiz S L, DeLapp K, Taiz L

Abstract excerpt

Carrot root cells were transformed with the coding or 5' noncoding regions of the carrot vacuolar H+ ATPase A subunit cDNA cloned in the antisense orientation behind the cauliflower mosaic virus 35S promoter. Bafilomycin-sensitive ATPase, H(+)-pumping, and 14C-O-methyl-glucose uptake activities were specifically inhibited in the tonoplast fractions of mutant cell lines. Protein gel blotting confirmed that the...

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Identifiers

PMID: 1392599
DOI: 10.1105/tpc.4.7.851
PMCID: PMC160180

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Source receipt: pubmed, retrieved 7 Sept 2026.

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