Article
The use of antisense mRNA to inhibit the tonoplast H+ ATPase in carrot.
The Plant cell - 1 Jul 1992
Gogarten J P, Fichmann J, Braun Y, Morgan L, Styles P, Taiz S L, DeLapp K, Taiz L
Abstract excerpt
Carrot root cells were transformed with the coding or 5' noncoding regions of the carrot vacuolar H+ ATPase A subunit cDNA cloned in the antisense orientation behind the cauliflower mosaic virus 35S promoter. Bafilomycin-sensitive ATPase, H(+)-pumping, and 14C-O-methyl-glucose uptake activities w...
Topics
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA, Antisense
- Electrophoresis, Gel, Two-Dimensional
- Molecular Sequence Data
- Phenotype
- Plants
- Polymerase Chain Reaction
- Proton-Translocating ATPases
- RNA, Antisense