Article
The effect of supercoiling on the in vitro transcription of the spoIIA operon from Bacillus subtilis.
Biochimie - 1 Jan 2000
Bird T, Burbulys D, Wu J J, Strauch M A, Hoch J A, Spiegelman G B
Abstract excerpt
The spoIIA operon codes for an alternative sigma factor which appears in the early stages of a sigma factor expression cascade during sporulation in Bacillus subtilis. We have used a single round in vitro transcription assay to probe requirements for transcription initiation at the spoIIA promote...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- DNA, Bacterial
- DNA, Superhelical
- DNA-Directed RNA Polymerases
- Electrophoresis, Polyacrylamide Gel
- Gene Expression
- Molecular Sequence Data
- Mutation
- Operon
- Promoter Regions, Genetic
- Sigma Factor
- Spores, Bacterial
- Transcription Factors
- Transcription, Genetic
- beta-Galactosidase