Article
The effect of supercoiling on the in vitro transcription of the spoIIA operon from Bacillus subtilis.
Biochimie - 1 Jan 2000
Bird T, Burbulys D, Wu J J, Strauch M A, Hoch J A, Spiegelman G B
Abstract excerpt
The spoIIA operon codes for an alternative sigma factor which appears in the early stages of a sigma factor expression cascade during sporulation in Bacillus subtilis. We have used a single round in vitro transcription assay to probe requirements for transcription initiation at the spoIIA promoter. Core RNA polymerase or holoenzyme containing sigma A was reconstituted with sigma H protein and used to transcribe...
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