Article
Construction and expression of diphtheria toxin-encoding gene derivatives in Escherichia coli.
Gene - 1 Jul 1992
Zdanovsky A G, Kulaeva O I, Yankovsky N K
Abstract excerpt
We reported earlier that in the periplasmic space of Escherichia coli, truncated derivatives of diphtheria toxin undergo limited proteolysis [Zdanovsky et al., Mol. Biol. 22 (1988) 1037-1293]. Here, we present data indicating that this proteolysis is reduced in cells bearing a mutation in the deg...
Topics
- Bacteriophage lambda
- Blotting, Western
- Cloning, Molecular
- Diphtheria Toxin
- Escherichia coli
- Lac Operon
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Protein Sorting Signals
- Recombinant Fusion Proteins