Article
Complementary DNA cloning for galactosyltransferase associated with tumor and determination of antigenic epitopes recognized by specific monoclonal antibodies.
Cancer research - 15 Nov 1992
Uejima T, Uemura M, Nozawa S, Narimatsu H
Abstract excerpt
The galactosyltransferase associated with tumor (GAT) was the name given to the isoenzyme that tends to polymerize resulting in slower moving in a nondenaturing polyacrylamide gel electrophoresis than normal (beta 1-4)galactosyltransferase (normal GalT). A complementary DNA (cDNA) library was constructed from a human ovarian cancer cell line, RMG-I, which secreted an amount of GAT into the culture supernatant and...
Topics
- Antibodies, Monoclonal
- Antibody Specificity
- Base Sequence
- Blotting, Northern
- DNA, Neoplasm
- Epitopes
- Escherichia coli
- Female
- Galactosyltransferases
- Gene Expression
- Humans