Plasmid cDNA-directed protein synthesis in a coupled eukaryotic<i>in vitro</i>transcription-translation system

Abstract excerpt

A system is described in which transcription of cDNA clones by bacteriophage T7 RNA polymerase is coupled to translation in the micrococcal nuclease treated rabbit reticulocyte lysate in a single reaction of coupled transcription-translation. The monovalent and divalent cation requirements for translation are dominant for optimum expression in this coupled system, so that transcription is relatively inefficient....

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