Article
Enzymatic synthesis of p-nitrophenyl alpha-maltoheptaoside by transglycosylation of maltohexaose-forming amylase.
Agricultural and biological chemistry - 1 Mar 1990
Ogawa K, Uejima O, Nakakuki T, Usui T, Kainuma K
Abstract excerpt
An extracellular maltohexaose-forming amylase [EC 3.2.1.98] from Klebsiella pneumoniae mutant is a normal hydrolytic enzyme that hydrolyzes short-chain amylose (DP = 23) to give about 40% maltohexaose. Transglycosylation from maltoheptaose to the 4-position of p-nitrophenyl alpha-glucoside was efficiently induced through the use of maltohexaose-forming amylase in an aqueous methanol solution. The enzyme...
Topics
- Chromogenic Compounds
- Glucosides
- Glycosides
- Glycosylation
- Kinetics
- Klebsiella pneumoniae
- Mutation
- Thermodynamics
- alpha-Glucosidases