Article
Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction.
Bio/technology (Nature Publishing Company) - 1 Mar 1992
Pen J, Molendijk L, Quax W J, Sijmons P C, van Ooyen A J, van den Elzen P J, Rietveld K, Hoekema A
Abstract excerpt
As a first example of the feasibility of producing industrial bulk enzymes in plants, we have expressed Bacillus licheniformis alpha-amylase in transgenic tobacco, and applied the seeds directly in starch liquification. The enzyme was properly secreted into the intercellular space, and maximum expression levels of about 0.3% of total soluble protein were obtained. No apparent effect of the presence of the enzyme...
Topics
- Bacillus
- Bacterial Proteins
- Extracellular Space
- Genetic Vectors
- Glycosylation
- Phenotype
- Plants, Genetically Modified
- Plants, Toxic
- Starch
- Nicotiana
- Transformation, Genetic