Article
[Genotype reliability of short tandem repeats typing from minute DNA].
Fa yi xue za zhi - 1 Jan 2003
Lu De-jian, Sun Hong-yu, Chen Li-xian
Abstract excerpt
OBJECTIVE: To understand the forensic practice of DNA profiling from minimal amount of DNA. METHODS: Serial dilutions of DNA were amplified with the PowerPlex 16 System Kit, then the genotyping of short tandem repeat(STR) was performed by ABI 377 DNA automated Sequencer. RESULTS: When the mount of DNA template was less than 250 pg, allelic drop-out apparently occurred at several loci. Other disturbed peaks, such...
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