[Genotype reliability of short tandem repeats typing from minute DNA].

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Article

[Genotype reliability of short tandem repeats typing from minute DNA].

Fa yi xue za zhi - 1 Jan 2003

Lu De-jian, Sun Hong-yu, Chen Li-xian

Abstract excerpt

OBJECTIVE: To understand the forensic practice of DNA profiling from minimal amount of DNA. METHODS: Serial dilutions of DNA were amplified with the PowerPlex 16 System Kit, then the genotyping of short tandem repeat(STR) was performed by ABI 377 DNA automated Sequencer. RESULTS: When the mount of DNA template was less than 250 pg, allelic drop-out apparently occurred at several loci. Other disturbed peaks, such...

Topics

Alleles
DNA
Forensic Medicine
Genotype
Humans
Male
Minisatellite Repeats
Sequence Analysis, DNA
Tandem Repeat Sequences

Source

Open in PubMed

Identifiers

PMID: 13677317

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