Article
Activation of ergot alkaloid biosynthesis in prototrophic isolates by Claviceps purpurea protoplast fusion.
Journal of biotechnology - 1 Oct 1991
Didek-Brumec M, Gaberc-Porekar V, Alacević M, Milicić S, Socic H
Abstract excerpt
Intraspecific protoplast fusions were carried out with active ergocornine-ergokryptine and inactive ergocristine Claviceps purpurea strains and vice versa. The isolated prototrophic strains from both types of crossings produced all three alkaloid types, showing that biosynthesis of distinct alkaloid was activated in an inactive partner strain. The prototrophic isolates were stable on minimal medium but they...
Topics
- Claviceps
- Culture Media
- Ergot Alkaloids
- Mutation
- Protoplasts
