Growth kinetics of Chinese hamster ovary cells in a compact loop bioreactor. 3. Selection and characterization of an anchorage-independent subline and medium improvement.

doi:10.1016/0168-1656(90)90040-i

Article

Growth kinetics of Chinese hamster ovary cells in a compact loop bioreactor. 3. Selection and characterization of an anchorage-independent subline and medium improvement.

Journal of biotechnology - 1 Nov 1990

Kurano N, Leist C, Messi F, Gandor C, Kurano S, Fiechter A

Abstract excerpt

Four sublines of Chinese hamster ovary (CHO) cells were selected or cloned on a 10% fetal calf serum supplemented MEM-alpha medium. Three of them were monolayer cultures and could proliferate by 2000 times a week (mu = 1.1 d 1) in T-flasks. The other subline, S1, could grow in suspension even in static T-flask cultures. The stability in chromosome number of these cell lines was investigated. By evaluating the...

Topics

Animals
Biotechnology
Cell Division
Cell Line
Chromosomes
Cricetinae
Culture Media
Female
Genetic Variation
Glucose
Kinetics

Growth kinetics of Chinese hamster ovary cells in a compact loop bioreactor. 3. Selection and characterization of an anchorage-independent subline and medium improvement.

Abstract excerpt

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