Article
Optimal chemostat cascades for periplasmic protein production.
Biotechnology progress - 1 Jan 2000
Davis R H, Ramirez W F, Chatterjee A
Abstract excerpt
This theoretical work predicts the optimal system design for the steady-state production of secreted protein in a chemostat cascade, using bakers' yeast (Saccharomyces cerevisiae) as the host organism. The protein of interest, mutant invertase, is secreted to the periplasmic space instead of the culture medium on account of its large size. This work uses the secretion model developed and tested by Park and...
Topics
- Cloning, Molecular
- Genetic Engineering
- Glycoside Hydrolases
- Kinetics
- Mutation
- Recombinant Proteins
- Saccharomyces cerevisiae
- beta-Fructofuranosidase