Article
Purification and characterization of NF-R1 that regulates the expression of the human multidrug resistance (MDR1) gene.
Nucleic acids research - 11 Nov 1992
Ogura M, Takatori T, Tsuruo T
Abstract excerpt
We have purified a protein, NF-R1, that specifically binds to two unrelated motifs--the ATTCAGTCA motif and the GC-box motif--on the MDR1 proximal promoter. Purified NF-R1 has been confirmed by southwestern blotting to be a 110-kDa protein. Methylation interference analysis revealed that the nucl...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Base Sequence
- Cells, Cultured
- DNA
- DNA-Binding Proteins
- Drug Resistance
- Gene Expression Regulation
- Humans
- Membrane Glycoproteins
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Protein Binding
- Transfection