Article
Purification and characterization of NF-R2 that regulates the expression of the human multidrug resistance (MDR1) gene.
Japanese journal of cancer research : Gann - 1 Mar 1993
Takatori T, Ogura M, Tsuruo T
Abstract excerpt
NF-R2 is a DNA-binding protein that interacts with the MDR1 gene proximal promoter sequence. We previously reported that NF-R2 binds within the promoter's -126 and -102 regions, which contain the ATTCAGTCA motif. In the present study, we have purified NF-R2 from the nuclear extract of K562/ADM ce...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Base Sequence
- Chloramphenicol O-Acetyltransferase
- Chromatography, Affinity
- Chromatography, Gel
- Chromatography, Ion Exchange
- Drug Resistance
- Electrophoresis, Polyacrylamide Gel
- Gene Expression Regulation, Neoplastic
- Humans
- Leukemia, Myeloid
- Membrane Glycoproteins
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Regulatory Sequences, Nucleic Acid
- Sequence Homology, Nucleic Acid