Article
Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors.
Science (New York, N.Y.) - 30 Oct 1992
Kallioniemi A, Kallioniemi O P, Sudar D, Rutovitz D, Gray J W, Waldman F, Pinkel D
Abstract excerpt
Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or...
Topics
- Chromosome Mapping
- DNA Probes
- DNA, Neoplasm
- Female
- Fluorescein-5-isothiocyanate
- Fluorescent Dyes
- Gene Amplification
- Gene Deletion
- Humans
- In Situ Hybridization, Fluorescence
- Male