Article
Genotypic screening of pseudorabies virus strains for thymidine kinase deletions by use of the polymerase chain reaction.
American journal of veterinary research - 1 Jun 1992
Dangler C A, Deaver R E, Kolodziej C M, Rupprecht J D
Abstract excerpt
Genetic recombination between field strains and vaccine strains of pseudorabies virus (PRV) has been suggested as a scenario that might arise from use of deletion-mutant modified-live vaccine strains, particularly those strains attenuated by deletions within the thymidine kinase (TK-) gene locus. To address this hypothesis experimentally, it is necessary to screen large numbers of PRV isolates for their TK...
Topics
- Animals
- Base Sequence
- DNA, Viral
- Deoxyribonucleases, Type II Site-Specific
- Genotype
- Herpesvirus 1, Suid
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Restriction Mapping
- Thymidine Kinase