Article
Cloning, expression, and sequence of an allosteric mutant ADPglucose pyrophosphorylase from Escherichia coli B.
Archives of biochemistry and biophysics - 1 Apr 1993
Meyer C R, Ghosh P, Nadler S, Preiss J
Abstract excerpt
Escherichia coli B mutant strain SG14 accumulates glycogen at 28% of the rate observed for the parent strain. This is due to the presence of an ADPglucose pyrophosphorylase with altered allosteric properties including lower apparent affinities for substrates, the activator, fructose-1,6-bisphosph...
Topics
- Adenosine Monophosphate
- Allosteric Regulation
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Fructosediphosphates
- Gene Expression
- Glucose-1-Phosphate Adenylyltransferase
- Kinetics
- Magnesium
- Molecular Sequence Data
- Mutation
- NADP
- Nucleic Acid Hybridization
- Nucleotidyltransferases
- Phosphoenolpyruvate
- Plasmids