Article
Cloning, expression, and nucleotide sequence of glgC gene from an allosteric mutant of Escherichia coli B.
Archives of biochemistry and biophysics - 1 Jul 1992
Ghosh P, Meyer C, Remy E, Peterson D, Preiss J
Abstract excerpt
The Escherichia coli B mutant strain CL1136 accumulates glycogen at a 3.4- to 4-fold greater rate than the parent E. coli B strain and contains an ADPglucose synthetase with altered kinetic and allosteric properties. The enzyme from CL1136 is less dependent on the allosteric activator, fructose 1...
Topics
- Allosteric Regulation
- Amino Acid Sequence
- Base Sequence
- Chromatography, Ion Exchange
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genomic Library
- Glucose-1-Phosphate Adenylyltransferase
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Recombinant Proteins
- Restriction Mapping