Article
Cloning of the outer membrane high-affinity Fe(III)-pyochelin receptor of Pseudomonas aeruginosa.
Journal of bacteriology - 1 Jul 1992
Ankenbauer R G
Abstract excerpt
Pseudomonas aeruginosa produces the phenolic siderophore pyochelin under iron-limiting conditions. In this study, an Fe(III)-pyochelin transport-negative (Fpt-) strain, IA613, was isolated and characterized. 55Fe(III)-pyochelin transport assays determined that no Fe(III)-pyochelin associated with the Fpt- IA613 cells while a significant amount associated with KCN-poisoned Fpt+ cells. A P. aeruginosa genomic...
Topics
- Bacterial Outer Membrane Proteins
- Cell Membrane
- Cloning, Molecular
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Iron
- Iron Chelating Agents
- Kinetics
- Molecular Weight
- Phenols