Article
Mutations in bglY, the structural gene for the DNA-binding protein H1 of Escherichia coli, increase the expression of the kanamycin resistance gene carried by plasmid pGR71.
Molecular & general genetics : MGG - 1 May 1992
Bertin P, Lejeune P, Colson C, Danchin A
Abstract excerpt
bglY mutants of Escherichia coli K12 which show higher levels of kanamycin resistance (Kmr) in the presence of plasmid pGR71 have been previously described. In this work, we show that this increased resistance to an aminoglycoside antibiotic is not due either to low drug uptake or to alteration of its target, the ribosome. The copy number of plasmid pGR71 is not modified. The fact that increased antibiotic...
Topics
- Aminoglycosides
- Anti-Bacterial Agents
- DNA, Bacterial
- Enzyme Stability
- Escherichia coli
- Genes, Bacterial
- Histones
- Hot Temperature
- Kanamycin Resistance
- Kinetics
- Microbial Sensitivity Tests