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Use of Loop-Mediated Isothermal Amplification of the IS<i>900</i>Sequence for Rapid Detection of Cultured<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>

1 Sept 2003

Abstract excerpt

We evaluated the usefulness of loop-mediated isothermal amplification (LAMP) in detecting specific gene sequences of Mycobacterium avium subsp. paratuberculosis (MAP). A total of 102 primer sets for LAMP was designed to amplify the IS900, HspX, and F57 gene sequences of MAP. Using each of two primer sets (P-1 and P-2) derived from the IS900 fragment, it was possible to detect MAP in a manner similar to that used...

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PMID: 12958269
DOI: 10.1128/jcm.41.9.4359-4365.2003

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