Article
Multistep autoactivation of asparaginyl endopeptidase in vitro and in vivo.
The Journal of biological chemistry - 3 Oct 2003
Li Dongtao Ni, Matthews Stephen P, Antoniou Antony N, Mazzeo Daniela, Watts Colin
Abstract excerpt
Mammalian asparaginyl endopeptidase (AEP) or legumain is a recently discovered lysosomal cysteine protease that specifically cleaves after asparagine residues. How this unusually specific lysosomal protease is itself activated is not fully understood. Using purified recombinant pro-enzyme, we show that activation is autocatalytic, requires sequential removal of C- and N-terminal pro-peptides at different pH...
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