Article
Multistep autoactivation of asparaginyl endopeptidase in vitro and in vivo.
The Journal of biological chemistry - 3 Oct 2003
Li Dongtao Ni, Matthews Stephen P, Antoniou Antony N, Mazzeo Daniela, Watts Colin
Abstract excerpt
Mammalian asparaginyl endopeptidase (AEP) or legumain is a recently discovered lysosomal cysteine protease that specifically cleaves after asparagine residues. How this unusually specific lysosomal protease is itself activated is not fully understood. Using purified recombinant pro-enzyme, we show that activation is autocatalytic, requires sequential removal of C- and N-terminal pro-peptides at different pH...
Topics
- Amino Acid Sequence
- Animals
- Asparagine
- Aspartic Acid
- CHO Cells
- Cell Line
- Cells, Cultured
- Cloning, Molecular
- Cricetinae
- Cysteine Endopeptidases
- Dendritic Cells