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Article

The conserved cis-Pro39 residue plays a crucial role in the proper positioning of the catalytic base Asp38 in ketosteroid isomerase from Comamonas testosteroni.

The Biochemical journal - 15 Oct 2003

Nam Gyu Hyun, Cha Sun-Shin, Yun Young Sung, Oh Yun Hee, Hong Bee Hak, Lee Heung-Soo, Choi Kwan Yong

Abstract excerpt

KSI (ketosteroid isomerase) from Comamonas testosteroni is a homodimeric enzyme that catalyses the allylic isomerization of Delta5-3-ketosteroids to their conjugated Delta4-isomers at a reaction rate equivalent to the diffusion-controlled limit. Based on the structural analysis of KSI at a high resolution, the conserved cis-Pro39 residue was proposed to be involved in the proper positioning of Asp38, a critical...

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Identifiers

PMID: 12852789
DOI: 10.1042/BJ20030263
PMCID: PMC1223686

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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