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Article

Structural double-mutant cycle analysis of a hydrogen bond network in ketosteroid isomerase from Pseudomonas putida biotype B.

The Biochemical journal - 15 Sept 2004

Jang Do Soo, Cha Hyung Jin, Cha Sun-Shin, Hong Bee Hak, Ha Nam-Chul, Lee Ja Young, Oh Byung-Ha, Lee Heung-Soo, Choi Kwan Yong

Abstract excerpt

KSI (ketosteroid isomerase) catalyses an allylic isomerization reaction at a diffusion-controlled rate. A hydrogen bond network, Asp(99).Water(504).Tyr(14).Tyr(55).Tyr(30), connects two critical catalytic residues, Tyr(14) and Asp(99), with Tyr(30), Tyr(55) and a water molecule in the highly apolar active site of the Pseudomonas putida KSI. In order to characterize the interactions among these amino acids in the...

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Identifiers

PMID: 15228388
DOI: 10.1042/BJ20031871
PMCID: PMC1133972

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Source receipt: pubmed, retrieved 7 Sept 2026.

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