Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A novel screening system for self-mRNA targeting proteins.

Journal of biochemistry - 1 Apr 2003

Ying Bei-Wen, Suzuki Tsutomu, Shimizu Yoshihiro, Ueda Takuya

Abstract excerpt

Here we describe the application of an in vitro translation system for genetic screening, to identify RNA-binding proteins that bind to their own mRNAs. It is a relatively novel system designed using an advanced cell-free translation system reconstructed with purified translational components. Due to the absence of nucleases and proteases, the complex of mRNA and nascent polypeptide synthesized in this system is...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Motifs
Share to Antibody Affinity
Share to Gene Expression Regulation, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Plasmids

Share to Protein Biosynthesis

Share to Ribosomal Proteins

Share to RNA-Binding Proteins

Share to RNA, Ribosomal

Source

Identifiers

PMID: 12761296
DOI: 10.1093/jb/mvg064

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A novel screening system for self-mRNA targeting proteins.PMID 12761296Read ↗

Molecular mimicry in translational regulation: the case of ribosomal protein S15.Semantic proximity Read ↗

Discovery and validation of novel and distinct RNA regulators for ribosomal protein S15 in diverse bacterial phyla.Semantic proximity Read ↗

Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions.Semantic proximity Read ↗

Modified peptide selection in vitro by introduction of a protein-RNA interaction.Semantic proximity Read ↗

Structural elements of rps0 mRNA involved in the modulation of translational initiation and regulation of E. coli ribosomal protein S15.Semantic proximity Read ↗

Interaction of C5 protein with RNA aptamers selected by SELEX.Semantic proximity Read ↗

Interaction of the Bacillus stearothermophilus ribosomal protein S15 with its 5'-translational operator mRNA.Semantic proximity Read ↗

Solid-phase translation and RNA-protein fusion: a novel approach for folding quality control and direct immobilization of proteins using anchored mRNA.Semantic proximity Read ↗

RNA regulators responding to ribosomal protein S15 are frequent in sequence space.Semantic proximity Read ↗

Target site of Escherichia coli ribosomal protein S15 on its messenger RNA. Conformation and interaction with the protein.Semantic proximity Read ↗

Molecular dissection of the pseudoknot governing the translational regulation of Escherichia coli ribosomal protein S15.Semantic proximity Read ↗

Functional characterization of ribosomal protein L15 from Saccharomyces cerevisiae.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.