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New method for the extraction of DNA from white blood cells for the detection of common genetic variants associated with thrombophilia.

Pathophysiology of haemostasis and thrombosis - 1 Jan 2000

Angelini Antonio, Di Febbo Concetta, Rullo Agnese, Di Ilio Carmine, Cuccurullo Franco, Porreca Ettore

Abstract excerpt

In the last few years, FV Leiden, prothrombin 20210 and thermolabile methylene tetrahydrofolate reductase (MTHFR) 677 variants have been identified as possible risk factors associated with thrombophilia, and many PCR-based methods have been described for detecting these variations. However, the genomic DNA extraction is still a rate-limiting and time-consuming step in the PCR process. In an attempt to accelerate...

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Identifiers

PMID: 12759519
DOI: 10.1159/000070424

Genes

MTHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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