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Article

Frequent coinfection with hepatitis B virus strains of distinct genotypes detected by hybridization with type-specific probes immobilized on a solid-phase support.

Journal of virological methods - 9 Jun 2003

Kato Hideaki, Orito Etsuro, Sugauchi Fuminaka, Ueda Ryuzo, Koshizaka Takuya, Yanaka Shoko, Gish Robert G, Kurbanov Fuat, Ruzibakiev Ruslan, Kramvis Anna, Kew Michael C, Ahmad Nooruddin, Khan Mobin, Usuda Sadakazu, Miyakawa Yuzo, Mizokami Masashi

Abstract excerpt

A genotype-specific probes assay (GSPA) was developed for distinguishing the seven genotypes (A-G) of hepatitis B virus (HBV). Nucleotide (nt) sequences corresponding to preS1 region were amplified by PCR with a primer labeled with biotin, and delivered to eight wells on which complementary sequences specific to one or other genotype had been immobilized. Thereafter, hybridization of HBV DNA sequences amplified...

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PMID: 12757918
DOI: 10.1016/s0166-0934(03)00095-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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