Article
Molecular beacons as diagnostic tools: technology and applications.
Clinical chemistry and laboratory medicine - 1 Apr 2003
Abravaya Klara, Huff Jeffrey, Marshall Ron, Merchant Barbara, Mullen Carolyn, Schneider George, Robinson John
Abstract excerpt
Molecular beacons are single-stranded, fluorophore-labeled nucleic acid probes that are capable of generating a fluorescent signal in the presence of target, but are dark in the absence of target. Molecular beacons allow multiplex detection of PCR products in real time in a homogeneous assay format. Real time detection is inherently quantitative and affords a greater dynamic range than end-point detection...
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