Article
Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase.
Molecular biotechnology - 1 Jun 2003
Igarashi Satoshi, Sode Koji
Abstract excerpt
Water-soluble quinoprotein glucose dehydrogenase (PQQGDH-B) is a dimeric enzyme whose application for glucose sensing is the focus of much attention. We attempted to increase the thermal stability of PQQGDH-B by introducing a disulfide bond at the dimer interface. The Ser residue at position 415 was selected for substitution with Cys, as structural information revealed that its side chains face each other at the...
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