Article
A new and versatile method for the successful conversion of AFLP markers into simple single locus markers.
Nucleic acids research - 15 May 2003
Brugmans Bart, van der Hulst Ron G M, Visser Richard G F, Lindhout Pim, van Eck Herman J
Abstract excerpt
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of AFLP markers from complex fingerprints into simple single locus assays is perceived as problematic because DNA sequence information is required for the design of new locus-specific PCR primers. In addition, single...
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