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Article

Generation of Ci-Brachyury-GFP stable transgenic lines in the ascidian Ciona savignyi.

Genesis (New York, N.Y. : 2000) - 1 Apr 2003

Deschet Karine, Nakatani Yuki, Smith William C

Abstract excerpt

We report generation of stable transgenic lines of the ascidian Ciona savignyi carrying a Ciona intestinalis-Brachyury-promoter/Green Fluorescent Protein-reporter (Ci-Bra-GFP) construct. The transgenic lines were made using a technique in which the endonuclease I-SceI was coinjected into fertilized eggs with a transgene construct containing flanking recognition sites for I-SceI. Two founder animals, out of 12...

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PMID: 12717736
DOI: 10.1002/gene.10195

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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