Article
Validation of a modified PCR-based method for identifying mutant restricted ovulator chickens: substantiation of genotypic classification by phenotypic traits.
Poultry science - 1 Apr 2003
Elkin R G, Zhong Y, Porter R E, Walzem R L
Abstract excerpt
Upon photostimulation, restricted ovulator (RO) female chickens exhibit endogenous hyperlipidemia, develop atherosclerotic lesions, and generally fail to lay eggs. This phenotype results from a point mutation in the gene specifying the very low density lipoprotein receptor (VLDLR), whose protein product normally mediates the massive oocytic uptake of egg yolk precursors from the circulation. Taking advantage of...
Topics
- Animals
- Base Sequence
- Chickens
- DNA
- Egg Proteins
- Egg Yolk
- Electrophoresis, Polyacrylamide Gel
- Female
- Gene Amplification
- Genotype
- Lipoproteins, VLDL
- Male
- Oviposition
- Phenotype
- Point Mutation
- Polymerase Chain Reaction
- Receptors, LDL
- Vitellogenins