Alkaline proteinase inhibitor of Pseudomonas aeruginosa: a mutational and molecular dynamics study of the role of N-terminal residues in the inhibition of Pseudomonas alkaline proteinase.

Abstract excerpt

Alkaline proteinase inhibitor of Pseudomonas aeruginosa is a 11.5-kDa, high affinity inhibitor of the serralysin class of zinc-dependent proteinases secreted by several Gram-negative bacteria. X-ray crystallography of the proteinase-inhibitor complex reveals that five N-terminal inhibitor residues occupy the extended substrate binding site of the enzyme and that the catalytic zinc is chelated by the alpha-amino...

Topics

• Amino Acids
• Catalytic Domain
• Circular Dichroism
• Crystallography, X-Ray
• Ions
• Kinetics
• Models, Molecular
• Mutation
• Plasmids
• Polymerase Chain Reaction
• Protease Inhibitors