Article
Detection of infectious bronchitis virus by multiplex polymerase chain reaction and sequence analysis.
Journal of virological methods - 1 Apr 2003
Liu Hung J, Lee Long H, Shih Wen L, Lin Maw Y, Liao Ming H
Abstract excerpt
A multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to amplify the S1 and S2 genes of vaccine and recent Taiwanese isolates of infectious bronchitis virus (IBV). DNA fragments of 228 and 400 base pairs in length were amplified among IBV isolates in multiplex PCR, suggesting that there were no apparent deletions or insertions in these regions. No PCR products were amplified from...
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