Article
Typing of field isolates of infectious bronchitis virus based on the sequence of the hypervariable region in the S1 gene.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Jul 2003
Lee Chang-Won, Hilt Deborah A, Jackwood Mark W
Abstract excerpt
A universal primer set was developed that amplifies a region covering hypervariable region (HVR) 1 and HVR 2 in the S1 gene of the infectious bronchitis virus (IBV). The universality of this primer set was confirmed by testing the reference strains of different serotypes or variants of the IBV present in the United States. An approximately 450-bp region containing HVR 1 and HVR 2 of 7 untyped field isolates...
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