Article
Detection of universal variable fragments as markers for genetic studies. A novel technology for DNA fingerprinting.
Molecular biotechnology - 1 Feb 2003
van Haeringen W A, van de Goor L H P, Panneman H, van Lith H A, van Haeringen H, van Zutphen L F M
Abstract excerpt
A novel DNA technology enables the detection of universal variable fragments (UVF), thus revealing genetic variation without a priori sequence information. The detection of UVF markers is based on two amplifications of genomic DNA with the polymerase chain reaction. In the first amplification, two short oligonucleotide primers produce a large number of fragments. One primer is based on a microsatellite sequence,...
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