Article
MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues.
Genome biology - 1 Jan 2003
Blackshaw Seth, Kuo Winston P, Park Peter J, Tsujikawa Motokazu, Gunnersen Jenny M, Scott Hamish S, Boon Wee-Ming, Tan Seong-Seng, Cepko Constance L
Abstract excerpt
BACKGROUND: Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate. RESULTS: We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals. CONCLUSIONS: MicroSAGE is a...
Topics
- 3T3 Cells
- Adult
- Age Factors
- Aged
- Aged, 80 and over
- Animals
- Cell Line
- Computers, Molecular
- Female
- Gene Expression Profiling
- Gene Library