Article
Hemin binding, functional expression, and complementation analysis of Pap 31 from Bartonella henselae.
Journal of bacteriology - 1 Mar 2003
Zimmermann Rainer, Kempf Volkhard A J, Schiltz Emile, Oberle Karin, Sander Anna
Abstract excerpt
Growth of Bartonella henselae is strongly heme dependent, and B. henselae is unable to synthesize heme itself. At least five outer membrane-associated proteins from B. henselae bind hemin, including the 31-kDa protein designated Pap31. The gene of this protein was heterologously expressed in Escherichia coli M15(pREP4) and detected with monoclonal antibodies in the outer membrane fraction. Complementation of the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
