Article
Import of Agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins.
The Plant cell - 1 Feb 2001
Ziemienowicz A, Merkle T, Schoumacher F, Hohn B, Rossi L
Abstract excerpt
To study the mechanism of nuclear import of T-DNA, complexes consisting of the virulence proteins VirD2 and VirE2 as well as single-stranded DNA (ssDNA) were tested for import into plant nuclei in vitro. Import of these complexes was fast and efficient and could be inhibited by a competitor, a nuclear localization signal (NLS) coupled to BSA. For import of short ssDNA, VirD2 was sufficient, whereas import of long...
Topics
- Active Transport, Cell Nucleus
- Bacterial Proteins
- Base Sequence
- DNA, Bacterial
- DNA, Single-Stranded
- DNA-Binding Proteins
- Genes, Bacterial
- Ion Channels
- Models, Biological
- Mutation
- Nuclear Localization Signals
- Plants, Genetically Modified
- Protein Binding
- Rec A Recombinases
- Rhizobium
- Nicotiana