[Sequence analyzing and genotyping of the gene encoding glutamate rich protein of geographically different Plasmodium falciparum isolates obtained from different malaria endemic areas].

Abstract excerpt

OBJECTIVE: To sequence a gene encoding GLURP and identify the genotypes of geographically different Plasmodium falciparum isolates from Yunnan and Hainan Provinces, China. METHODS: The gene of R2 repeat region of GLURP was amplified by the nested polymerase chain reaction and cloned into T-vector. The nucleotide sequence of the GLURP gene was determined using automatic sequencer (dideoxy chain termination...

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