Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Identification of regions of the tomato gamma-glutamyl kinase that are involved in allosteric regulation by proline.

The Journal of biological chemistry - 18 Apr 2003

Fujita Tomomichi, Maggio Albino, Garcia-Rios Mario, Stauffacher Cynthia, Bressan Ray A, Csonka Laszlo N

Abstract excerpt

The first step of proline biosynthesis is catalyzed by gamma-glutamyl kinase (GK). To better understand the feedback inhibition properties of GK, we randomly mutagenized a plasmid carrying tomato tomPRO1 cDNA, which encodes proline-sensitive GK. A pool of mutagenized plasmids was transformed into an Escherichia coli GK mutant, and proline-overproducing derivatives were selected on minimal medium containing the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Allosteric Site
Share to Crystallography, X-Ray

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Dimerization

Share to Dose-Response Relationship, Drug

Share to Gene Expression Regulation, Enzymologic

Share to Peptides

Share to Proline

Share to Solanum lycopersicum

Source

Identifiers

PMID: 12566437
DOI: 10.1074/jbc.M212177200

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Identification of regions of the tomato gamma-glutamyl kinase that are involved in allosteric regulation by proline.PMID 12566437Read ↗

Desensitization of feedback inhibition of the Saccharomyces cerevisiae gamma-glutamyl kinase enhances proline accumulation and freezing tolerance.Semantic proximity Read ↗

Elevation of free proline and proline-rich protein levels by simultaneous manipulations of proline biosynthesis and degradation in plants.Semantic proximity Read ↗

Isolation of baker's yeast mutants with proline accumulation that showed enhanced tolerance to baking-associated stresses.Semantic proximity Read ↗

Proline antagonizes GABA-induced quenching of quorum-sensing in Agrobacterium tumefaciens.Semantic proximity Read ↗

Mutations in the listerial proB gene leading to proline overproduction: effects on salt tolerance and murine infection.Semantic proximity Read ↗

Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase.Semantic proximity Read ↗

Does proline accumulation play an active role in stress-induced growth reduction?Semantic proximity Read ↗

Effect of single base substitutions at glycine-870 codon of gramicidin S synthetase 2 gene on proline activation.Semantic proximity Read ↗

Glycine betaine-assisted protein folding in a lysA mutant of Escherichia coli.Semantic proximity Read ↗

Regulation of the glyoxylate bypass operon: cloning and characterization of iclR.Semantic proximity Read ↗

Defining the cytosolic pathway of glutathione degradation in Arabidopsis thaliana: role of the ChaC/GCG family of γ-glutamyl cyclotransferases as glutathione-degrading enzymes and AtLAP1 as the Cys-Gly peptidase.Semantic proximity Read ↗

Proline transport and stress tolerance of ammonia-insensitive mutants of the PUT4-encoded proline-specific permease in yeast.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.