Article
In vivo import experiments in protoplasts reveal the importance of the overall context but not specific amino acid residues of the transit peptide during import into chloroplasts.
Molecules and cells - 31 Dec 2002
Lee Kwang Hee, Kim Dae Heon, Lee Sang Woo, Kim Zhoo Hyeon, Hwang Inhwan
Abstract excerpt
The N-terminal transit peptide of chloroplast proteins is necessary and sufficient to direct proteins to the chloroplasts. However, the requirement of the transit peptide of chloroplast proteins is not fully understood. In this study we investigated the requirement of a transit peptide at the level of amino acid sequence using an in vivo targeting approach. Targeting experiments with green fluorescent protein...
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