Article
Large-scale translocation reversal within the thylakoid Tat system in vivo.
The Journal of cell biology - 24 Oct 2005
Di Cola Alessandra, Robinson Colin
Abstract excerpt
In vitro import assays have shown that the thylakoid twin-arginine translocase (Tat) system transports folded proteins in a unidirectional manner. Here, we expressed a natural substrate, pre-23K, and a 23K presequence-green fluorescent protein (GFP) chimera in vivo in tobacco protoplasts. Both are imported into chloroplasts, targeted to the thylakoids, and processed to the mature size by the lumen-facing...
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