Article
Functional domains of the ferredoxin transit sequence involved in chloroplast import.
The Journal of biological chemistry - 24 Feb 1995
Pilon M, Wienk H, Sips W, de Swaaf M, Talboom I, van 't Hof R, de Korte-Kool G, Demel R, Weisbeek P, de Kruijff B
Abstract excerpt
In order to analyze the information content of a chloroplast transit sequence, we have constructed and analyzed by in vitro assays seven substitution and 20 deletion mutants of the ferredoxin transit sequence. The N-terminal part and the C-terminal part are important for targeting, and in addition the C-terminal region is required for processing. A third region is important for translocation but not for the...
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