Article
Functional domains of the ferredoxin transit sequence involved in chloroplast import.
The Journal of biological chemistry - 24 Feb 1995
Pilon M, Wienk H, Sips W, de Swaaf M, Talboom I, van 't Hof R, de Korte-Kool G, Demel R, Weisbeek P, de Kruijff B
Abstract excerpt
In order to analyze the information content of a chloroplast transit sequence, we have constructed and analyzed by in vitro assays seven substitution and 20 deletion mutants of the ferredoxin transit sequence. The N-terminal part and the C-terminal part are important for targeting, and in addition the C-terminal region is required for processing. A third region is important for translocation but not for the...
Topics
- Amino Acid Sequence
- Base Sequence
- Biological Transport
- Chloroplasts
- Ferredoxins
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Protein Processing, Post-Translational
- Sequence Deletion