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Article

Affinity labeling of cysteine-mutants evidences contact residues in modeled receptor binding sites.

Journal of receptor and signal transduction research - 1 Jan 2000

Perret P, Laube B, Schemm R, Betz H, Goeldner M, Foucaud B

Abstract excerpt

To investigate the topology of binding sites in two ionotropic receptors, we have initiated a strategy combining affinity labeling with cysteine-scanning mutagenesis. For the GABAA receptor we have used reactive derivatives of non-competitive blockers (NCBs) to explore interacting positions in its channel. The polypeptide positions of the M2 segment of the alpha1 subunit which we mutated into cysteine were...

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PMID: 12503626
DOI: 10.1081/rrs-120014606

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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